3.3. miRNA Microarrays. Microarrays represent a high-throughput method used to concurrently detect presence and changes in levels of a wide range of miRNAs in an individual experiment. Microarrays are based on nucleic acid hybridization between target molecules and their corresponding complementary probes.

Microarrays can compare levels of miRNA expression in different organs, tissues and species, and are a major, high throughput tool to analyze hundreds of miRNAs simultaneously. Sensitivity of microarrays is relatively low, mostly due to the amount of miRNA applied to the array.

GeneChip™ miRNA Arrays are powerful tools for studying the role of small non-coding RNA (miRNA, snoRNA, and scaRNA) in complex diseases, such as cancer. Perform comprehensive miRNA profiling from as liitle as 130 ng and start exploring the role of miRNA in 24 hours. Reagent required to generate miRNA expression profiles using GeneChip miRNA arrays.

Dec 12, 2018 · MicroRNAs (miRNAs) are evolutionary conserved, ~18–24 nucleotides long non-coding RNA, playing a significant role in controlling human gene expression by post-transcriptional gene regulation or...

Feb 1, 2008 · miRNA microarrays have been successfully used for the primary identification of new miRNAs that were predicted by bioinformatics approaches, for the dissection of differential expression of different miRNAs in the same cells, and for the comparison of miRNA expression profiles from different tissues or cells.

Mar 13, 2008 · Microarray technology is a powerful high-throughput tool capable of monitoring the expression of thousands of small noncoding RNAs at once within tens of samples processed in parallel in a single...

Jan 9, 2009 · Several methodologies, including cloning, northern blotting, real-time RT-PCR, microRNA arrays and ISH (in situ hybridization), have been developed and applied successfully in miRNA profiling. This review discusses the main existing microRNA detection technologies, while emphasizing microRNA arrays.

Apr 18, 2012 · MicroRNAs (miRNAs) are small RNAs (∼ 22 nucleotides long) that post-transcriptionally regulate the expression of thousands of genes in a broad range of organisms.

We analyzed three biological samples across six miRNA microarray platforms and compared their hybridization performance. We examined the utility of these platforms, as well as NGS, for the detection of differentially expressed miRNAs.

To conduct a genome-wide analysis of miRNA expression of normal and disease samples, such as cancer, and to distinguish expression signatures associated with diagnosis, prognosis and therapeutic interventions, we have developed a unique …