Quantitative PCR (qPCR): A molecular technique that amplifies and quantifies target DNA sequences in real time, enabling rapid detection and enumeration.

Background Chronic inflammation and elevated reactive oxygen species are key contributors to hepatocellular carcinoma (HCC) ...

Among the available methods, real-time quantitative PCR (qPCR) is the most sensitive, capable of detecting DNA at picogram (10-12 g) concentrations. qPCR also enables multiplexing, simultaneously ...

Whether antiretroviral therapy (ART) is always completely suppressive, or HIV might continue to replicate at low levels despite ART in some people with HIV (PWH), is still debated. Here, we ...

Reverse transcription polymerase chain reaction (RTPCR) is a technique to detect or quantify RNA. It uses a reverse transcriptase enzyme to convert RNA to DNA, followed by PCR to amplify the DNA.

The data-driven approach has become significant in various scientific fields, such as climate modeling and weather forecasting, where a mechanistic description of physics and chemistry is either ...

Facing the prevalence of mental disorders around the world, the burden of healthcare services becomes increasingly imminent. To lessen patients' suffering, the timely diagnosis and therapy of mental ...

PCRs have revolutionised the detection of bacteria in clinical samples since their widespread introduction in the 1990s.1 Quantitative PCR (qPCR), also known as specific PCR, involves the targeting of ...

Abstract A real-time polymerase chain reaction (PCR) assay with fluorescence resonance energy transfer (FRET) hybridisation probes combined with melting curve analysis was developed to detect ...

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