We often use the term “definitive phenotypes” in flow cytometry, which describes the least number of markers to define specific cell populations. Not too long ago, flow cytometry assays were limited ...

The necessary blood volume was processed to obtain a minimum of 1 × 10 9 leukocytes without previous administration of colony-stimulating factors. Peripheral blood and the MNC product were quantified ...

We analyzed the expression of common intracellular molecules in CD4 + memory-like T cells by sorting CD45RO + CCR7 + CD4 + T cells from placental samples by flow cytometry. Higher levels of IL-17A and ...

Background The human enthesis conventional T cells are poorly characterised. Objectives To study the biology of the conventional T cells in human enthesis. Methods CD4+ and CD8+ T cells were ...

ABSTRACT Flow cytometry (FC) is an essential tool for diagnosis, prognosis and therapeutic follow-up of several hematologic malignancies. In addition, it performs the quantification of lymphocytes ...

Flow cytometry revealed cell purity of >95% after all separations (data not shown). Prior to coculture, purified CD4+ T cells were labeled with CFSE, then mixed with sorted PMN-MDSCs (4:1 ratio) in ...

Abstract CD4 + T cell activation is driven by 5-module receptor complexes. The T cell receptor (TCR) is the receptor module that binds composite surfaces of peptide antigens embedded within MHCII ...

Other than that, the use of flow cytometry was proven crucial for assessing the prognosis and lineage origin of myelodysplastic syndromes (MDS) [4]. The assessment of lymphocyte subpopulations, ...

The flow cytometry immunophenotyping of the peripheral blood was used to assess CD3+CD4+, CD3+CD8+, CD16+CD56+ and CD19+ counts obtained 100 days after the HSCT (± 14 days). The recovery of subtypes ...

In this article, Sartorius demonstrates how their iQue® can be used to reliably and accurately screen for memory T cell frequency through an optimized, multiplex flow cytometry assay.