In silico–designed nucleic acid probes and primers often do not achieve favorable specificity and sensitivity tradeoffs on the first try, and iterative empirical sequence-based optimization is ...

Locked nucleic acid (LNA) probes are modified RNA bases where the ribose is locked, thus fixing a specific conformation. They enable shorter probes for qPCR, thereby improving assay sensitivity ...

Luo, J.D. et al. Detection of rare mutant K-ras DNA in a single-tube reaction using peptide nucleic acid as both PCR clamp and sensor probe. Nucleic Acids Res. 34 , e12 (2006). Article Google Scholar ...

Five years ago, the NCBI Probe database (ProbeDB) was established to provide a centralized archive of molecular probes used in biomedical applications. Currently ProbeDB contains around 10 million ...

First, a peptide nucleic acid (PNA) probe locally opens a chosen target site, which allows a padlock DNA probe to access the site and become ligated. Second, rolling circle amplification ...

The use of nucleic acid amplification tests of “minipools” of 16 samples to screen blood donors for West Nile virus RNA began in July 2003. We report the yield and characteristics of positive ...

The rapid identification of respiratory virus outbreaks is needed to enable rational and effective public health interventions. We developed new quantitative approaches for simultaneous ...

SIDEBAR: Selected Suppliers of DNA and RNA Probes In the doctor's office or the research lab, the supreme value of DNA and RNA as diagnostic tools and the high potential of genetic therapy are putting ...

A team of researchers have developed an easy-to-use colorimetric assay for the detection of food contaminated with salmonella. The assay is based on a novel nucleic acid probe that is cleaved by ...

When one is detecting nucleic acids by hybridization, there are numerous situations in which it is not desirable to isolate probe-target complexes from the excess of probes that are used. Sanjay Tyagi ...

NUCLEIC ACID AMPLIFICATION AND DETECTION METHODS developed in the past decade are useful for the diagnosis and management of a variety of infectious diseases. The most widely used of these methods is ...

Biotinylated nucleotides have emerged as a powerful tool in molecular biology, enabling researchers to explore genetic materials with unprecedented precision and sensitivity. These modified ...