Research led by the Department of General and Visceral Surgery, Faculty of Medicine, University of Freiburg, Germany has ...

B cells and pDCs were identified by flow cytometry by gating on CD19/CD27 and blood dendritic cell antigen 2/CD123, respectively. Quilizumab, a mouse anti-human monoclonal IgG1 antibody with ...

Despite how widely flow cytometry is used with humanized mice, there are no standardized protocols, ... In order to fully understand the immune cell composition of a humanized mouse, ...

During this time, I learned various techniques, including animal handling, mouse preparation, staining for differentiation assays, flow cytometry, and maintaining mice and cell lines. I particularly ...

Figure 5 Calculated medians with interquartile ranges (IQRs) of immune cells quantified by flow cytometry within wild-type/control mouse brains. Medians with IQRs (defined as: 75 th percentile upper ...

Placental explants were dissected from the villous tissue and enzymatically digested to obtain a single cell suspension for flow cytometry (Figure 6 ... CD144-BV421 was visualised by the addition of ...

To this end, mouse BW5147 cells were transduced as described previously via lentiviral transduction (Halenius et al, 2011; Corrales‐Aguilar et al, 2013; Van den Hoecke et al, 2017). Human FcγR ...

It is extremely difficult to isolate a million cells from a rare cell population by flow cytometry. One study isolated over 1 million CD34 - Flt3 - Lineage - Sca-1 + c-kit + hematopoietic stem cells ...

Sources. Legroux, L. et al. (2015) An optimized method to process mouse CNS to simultaneously analyze neural cells and leukocytes by flow cytometry. Journal of Neuroscience Methods https://doi.org ...