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Surface plasmon resonance imaging (SPRi) brings label-free detection of molecular interactions to microarray analysis. The arrayed molecules (probes) may be proteins, peptides, nucleic acids ...
The following are some of the label-free protein microarray detection methods. These methods provide more direct information as they make use of innate proteins and ligands. Mass spectrometry ...
A microarray is a miniaturized assay comprising a solid surface printed with a multitude of molecules which are available for multiplex interaction and detection. They have applications in many fields ...
One way they do this is to use a DNA microarray to determine the expression levels of genes. When a gene is expressed in a cell, it generates messenger RNA (mRNA). Overexpressed genes generate ...
The microarray can then be scanned by a laser in order to detect the fluorescent label of bound cDNA molecules. The laser 'lights up' the fluorescently-labeled cDNA, marking the location of the ...
the sample is hybridized to the zip-code microarray, separating the individual molecules to known positions on the chip. This means that the incorporated label can be scored for each primer ...
In fact, microarray analysis enables researchers to ... convert it into complementary DNA (cDNA), and label the cDNA with a fluorescent dye. The fluorescently labeled cDNA is then added to a ...
A microarray is a miniaturized assay comprising a solid surface printed with a multitude of molecules which are available for multiplex interaction and detection. They have applications in many fields ...