Immunoprecipitation and immunoblotting were used to detect the expression of TNIP1, CCNG1, CCND1, and related proteins. A dual-luciferase reporter assay was employed to explore the molecular ...

Apoptotic/cell death markers were evaluated by flow cytometry. qPCR and immunoblotting were applied to quantify gene and protein expression. Results miR-181a-5p expression was increased in human FJ OA ...

Aging is the time-dependent process that all living organisms go through characterized by declining physiological function due to alterations in metabolic and molecular pathways. Many decades of ...

Correspondence to Dr Mirko Manetti, Department of Experimental and Clinical Medicine, Section of Anatomy and Histology, University of Florence, Largo Brambilla 3, Florence 50134, Italy; ...

(A) Analysis of interaction between Rudhira, Smad2/3 and MTs by co-immunoprecipitation and immunoblotting, as indicated (N= 3 independent experiments). (B) Loss of Smad-MT interaction on TGFβ ...

Scale bars, 5μm. (C) Immunoblotting assay revealed that DNAH12 was absent in the spermatozoa from P9, P10, and P11 harboring DNAH12 variants. β-actin was used as a loading control. (D) Sperm cells ...

In a paper published in Science Bulletin, a Chinese team of scientists led by Professor Bao revealed that the ...

Department of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital, Nanjing Medical University; State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral ...

Swathi Kaliki, MD, received the Ludwig von Sallmann Clinician-Scientist Award for her contributions to ophthalmology and ocular oncology. Kaliki leads the Operation Eyesight Universal Institute for ...

Figure 3. Representative images of the E. coli proteome array assays with the Dylight™ 549 fluorescence-labeled Prc protein variants Prc (WT), K455A-Prc (K455A), and ΔPDZ-K455A-Prc (ΔPDZ-K455A). Among ...