Engineering the replication of target DNA through cloning, or changing its genetic code through mutations, are detail-oriented processes whose foibles can spell disaster. Caitlin Smith looks at ...

Step 1: Extract DNA from a donor Image used with permission by copyright holder. ... Gene cloning is far more advantageous when it comes to healthcare and profit, and much easier to accomplish.

Many of the steps involved at this stage, such as PCR amplification, DNA restriction and ligation, clone screening, and sequencing, require specific clone design and a different set of restriction ...

Researchers in South Korea have made the process of creating embryonic stem cells through the cloning of human cells far more efficient than scientists previously believed was possible. The ...

New England Biolabs announces the introduction of the NEBuilder HiFi DNA Assembly Cloning Kit and Master Mix for one-step cloning and multiple DNA fragment assembly in as little as 15 minutes.

When Bull 86 died in 1997, scientists thought his unique genetic makeup was lost. But researchers at Texas A&M University were able to clone him from the frozen cells in 2000.

“This is also the first full de novo assembly of a human genome leveraging intact long native DNA (> 150 kb), without any clone libraries and the artifacts that cloning can introduce.” ...

Figure 1: One-step large-gene-cluster cloning by CATCH. After in-gel lysis of bacterial cells, the chromosomes are cleaved by RNA-guided Cas9 at the designated target sites.