Design primers to amplify target gene (ORF) 3. Amplify ORF via PCR 4.Ligate into pCR2,1-TOPO 5. Ligate into pJP10. Verify and sequence clone; ... The figure is a flow chart with 7 stages of action. 3 ...

This sample plan and flow chart is suitable for undergraduate students who are ready for calculus and starting in: 2024-25; 2023–24; 2022–23; 2021–22. In fall of ... Introduction to state space ...