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News-Medical.Net on MSNWeight loss wipes out senescent fat cells but leaves immune scars, study findsResearchers have mapped human fat tissue at single-cell resolution, uncovering how obesity alters cellular stress, ...
The team stained the osteocytes with DAPI, a solution that glows blue when it interacts with DNA. Lo and behold, some of the nucleus-like regions turned blue once the stain was applied.
DAPI fluorescence staining was used to calculate the amount of DNA in a nucleus and, subsequently, infer its cell cycle status, through a k -means clustering approach.
DAPI, or 4,6-diamidino2-phenylindole, is a fluorescent nucleic acid dye used to preferentially stain double-stranded DNA. It is commonly used in flow cytometry ...
Cellular viability is usually determined by measuring the capacity of cells to exclude vital dyes such as 4',6-diamidino-2-phenylindole (DAPI), or by assessing nuclear morphology with chromatinophilic ...
DNA content may be measured in association with other nuclear or cellular components such as cyclins by dual fluorescence analysis in which PI/DAPI is used to stain the DNA and for example FITC or APC ...
This image highlights the presence of regenerative cell populations adjacent to the damaged regions. Nuclear staining was performed using DAPI (blue).
Bio-Rad Laboratories, Inc. has announced the launch of its PureBlu™ Nuclear Staining Dyes, high purity formulations that simplify nuclear staining for fluorescence imaging experiments. In cell imaging ...
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