Compensation in flow cytometry is poorly understood ... In the absence of an adequate number of events, this calculation will not be accurate, and the compensation will suffer.

Mª José Marco from the Department of Hematology at Hopsital Dr. Peset talks to News-Medical Life Sciences about high complexity flow ... software to calculate the compensation matrix.

The experimental design of a multicolour flow ... compensation is carried out using single colour compensation tubes using BD Compensation Beads. This is covered in detail in the Compensation Section.

and in the CytoFLEX flow cytometer platform Beckman Coulter Life Sciences has calibrated them to have a linear response, which means that unlike PMTs, the compensation matrix obtained at one gain ...