In this article, we review recent developments in 2D HPLC and highlight variations in reversed-phase HPLC separation selectivity that allow for superior peak capacity in peptide fractionation.

Thermo Fisher has developed a two-dimensional high-performance liquid chromatography (2D-HPLC) method for determination of water-soluble vitamins in a complex multivitamin/mineral nutritional drink.

Modern 2D HPLC peptide separation protocols are designed to provide superior peak capacity through increased separation efficiency and orthogonality. Selecting the optimal fraction concatenation ...