The cutting and joining manipulations that underlies gene cloning are carried out by enzymes called restriction ... You will also be given some plasmid prepared by your TA to use as a positive control ...

After two months of fruitless effort, I was at my wit's end until a colleague suggested I try the TOPO TA Cloning® Kit from Invitrogen. "Look," he said, "all you need to do is to combine 1 microliter ...

Engineering the replication of target DNA through cloning, or changing its genetic code through mutations, are detail-oriented processes whose foibles can spell disaster. Caitlin Smith looks at ...

To generate the hairpin primer, select a 'sense' sequence(s) of 22 nucleotides (nt) in length from the coding sequence of the gene of interest ... miR-30 shRNA cloning experiments with reasonable ...