However, Koh's newly synthesized substrate retains a short hemicellulose tail attached to the reaction site, and it turned out that it is this tail that the enzyme binds to when performing its role.

More information: Michalis Chatzittofi et al, Mechanistic rules for de novo design of enzymes, Chem Catalysis (2025). DOI: 10.1016/j.checat.2025.101394 Provided by Max Planck Society ...

The formulae for calculating enzyme activity or substrate concentration are given below. Figure 5A shows the factor which is measured from the molar absorption coefficient for NADPH (6.32 * 103L ...