Although the LUNAâ„¢ can detect and count unstained cells, viability information can only be obtained from stained cell solutions. The traditional trypan blue can be used with the LUNAâ„¢, as well ...
However, functional analysis and in vivo transfer of MHC multimer-stained cells is hampered by the persistence of T-cell receptor (TCR)–MHC interactions and subsequently induced signaling events.
A trained neural network model processes a 3D axial stack of darkfield microscopy images, rapidly processing the 3D optical scattering information of bacterial cells to digitally stain label-free ...
Although the LUNA-IIâ„¢ can detect and count unstained cells, viability information can only be obtained from stained cell solutions. The traditional trypan blue can be used with the LUNA-II ...
For instance, you can add 25ml of a 2´ stock of stain to 25 ml of a 2´ stock of cells for a total volume of 50ml. All stainings are carried out at 4°C. Sometimes, higher intensity tetramer stains are ...
Confocal fluorescence microscopy (CFM) is widely used to make high-resolution cellular images but requires fluorescent staining, which poses risks of photobleaching or phototoxicity, potentially ...