Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size. In gel electrophoresis, the molecules to be separated are pushed by an ...

Two-dimensional polyacrylamide gel electrophoresis (PAGE ... Isoelectric focusing (IEF) separates proteins in a pH gradient. Each protein is 'focused' because it moves under the influence of ...

Sample preparation, including protein extraction and quantification Protein separation via gel electrophoresis Membrane transfer of proteins from the gel Immunoblotting with primary and secondary ...

Prior to mass spectrometry analysis, proteins are often separated to reduce sample complexity and improve detection. Common separation techniques include two-dimensional gel electrophoresis (2D-GE), ...