During the early stages of this science, a DNA test was performed using an analysis called restriction fragment length polymorphism. Because this process was extremely time consuming and required ...

“At the start, restriction enzymes were very important in isolating DNA fragments. These days, they’re more often used to diagnose whether you made the recombinant DNA properly or not,” Roberts said. ...

AFLP-PCR uses many of the same steps as RFLP, SSR, and RAPD, including restriction enzyme digestion of genomic DNA, PCR, and electrophoretic separation of DNA fragments. However, this method ...

The enzyme-specific sequence allows the ligation of the adapters to the resulting restriction fragments (green) without restoring the original restriction sites. In this way, ligated adapters ...