There are many popular chemistries for real-time PCR. One class uses different fluorescent dyes incorporated in short oligonucleotide probes specific for the amplified target. The second class ...

Thanks to the large array of dyes they offer, you can develop the ideal PCR or qPCR probe for your application without losing performance. Each dye is carefully paired with the correct BHQ ...

is an extension of traditional PCR, where DNA amplification over time is quantitated by measuring fluorescence from dyes intercalating into nascent DNA strands or by cleavage of target-specific probes ...

What is PCR? The key element of PCR is heat ... This refinement involves the use of fluorescent dyes or probes that label double-stranded DNA molecules. These fluorescent markers bind to the ...

Expert Rev Mol Diagn. 2012;12(7):731-754. SYBR Green and dual-hybridization probes are often used for melting point analysis at the end of the amplification. qPCR presents a number of advantages ...

Both the Mustang Purple TM passive reference dye-containing mix and the NO ROX mix can be used with TaqMan probes that contain ... master mixes have 72-hour pre-PCR benchtop stability.