In silico–designed nucleic acid probes and primers often do not achieve favorable specificity and sensitivity tradeoffs on the first try, and iterative empirical sequence-based optimization is ...

Locked nucleic acid (LNA) probes are modified RNA bases where the ribose is locked, thus fixing a specific conformation. They enable shorter probes for qPCR, thereby improving assay sensitivity ...

Fluorescent probes have emerged as indispensable tools in the detection and imaging of nucleic acids, underscoring advances in both fundamental research and clinical diagnostics.

Five years ago, the NCBI Probe database (ProbeDB) was established to provide a centralized archive of molecular probes used in biomedical applications. Currently ProbeDB contains around 10 million ...

First, a peptide nucleic acid (PNA) probe locally opens a chosen target site, which allows a padlock DNA probe to access the site and become ligated. Second, rolling circle amplification ...

SIDEBAR: Selected Suppliers of DNA and RNA Probes In the doctor's office or the research lab, the supreme value of DNA and RNA as diagnostic tools and the high potential of genetic therapy are putting ...

When one is detecting nucleic acids by hybridization, there are numerous situations in which it is not desirable to isolate probe-target complexes from the excess of probes that are used. Sanjay Tyagi ...

A team of researchers have developed an easy-to-use colorimetric assay for the detection of food contaminated with salmonella. The assay is based on a novel nucleic acid probe that is cleaved by ...

The use of nucleic acid amplification tests of “minipools” of 16 samples to screen blood donors for West Nile virus RNA began in July 2003. We report the yield and characteristics of positive ...

Rates did not differ significantly between users of the Gen-Probe nucleic acid–amplification test and users of the Roche test (P=0.74, data not shown). Table 1.

NUCLEIC ACID AMPLIFICATION AND DETECTION METHODS developed in the past decade are useful for the diagnosis and management of a variety of infectious diseases. The most widely used of these methods is ...