This article outlines how ITC provides real-time, enzyme kinetics data, in comparison to alternative enzyme assays. In part 2, how to perform enzyme kinetics investigations with a MicroCal ITC system ...

Real-time measurement of the initial stage of an enzyme–substrate reaction is essential for reproducible enzyme kinetics data. Automated direct-flow injection systems couple with various detection and ...

The results clearly demonstrate the advantage of using ITC for quantitative enzyme kinetics. For the given system, the ITC assay data correlates well with normal spectroscopic assay data, ...

The Post group has worked to understand the basis of the catalytic power of enzymes using information from crystallography, kinetics, and a description of conformational distributions from molecular ...