Real-time measurement of the initial stage of an enzyme–substrate reaction is essential for reproducible enzyme kinetics data. Automated direct-flow injection systems couple with various detection and ...

Enzyme activity is tightly regulated in living organisms to ... the behavior of enzymes under different conditions and can be used to determine important kinetic parameters, such as V max and K M. As ...

On the BioSpectrometer kinetic, the cuvette shaft can also be tempered in a range of +20 °C to +42 °C, meaning that enzyme and substrate kinetics can be measured directly in the device.

As the temperature increases, the kinetic energy increases, leading to more collisions and enzyme substrate complexes formed per unit time. This increases the rate of reaction. At the optimum ...