The basic scheme here is known as confocal laser scanning fluorescence microscopy, where a laser at one wavelength excites fluorescent tags bound to structures in a sample. Light emitted by the ...

but traditional confocal microscopy is limited in the range of wavelengths that can be covered and the number of fluorophores that can be used. Download this application note from Leica Microsystems ...

Scientists can now use super-resolution microscopy to directly observe living subcellular structures and activities. In this piece, we explore the basics of three popular super-resolution techniques.

As [Zachary Tong] points out, confocal scanning microscopy uses a clever optical setup to collect light from a single, well-defined point within a sample; rather than getting an image of all the ...

The Confocal Microscopy and Image Analysis Core provides instrumentation and expertise to support the acquisition, processing and analysis of both fluorescence and bright-field micrographs captured ...

Current concepts in light and electron microscopy and scanning probe techniques. Theory and practice of fluorescence (including confocal and multi-photon), atomic force, scanning and transmission ...

2 scanning electron microscopes (SEM), 2 transmission electron microscopes (TEM), 2 laser scanning confocal microscopes,1 deconvolution light microscope, 1 wide-field multimode light microscope, and 1 ...

The confocal microscope is equipped with 4 detectors, one of which is a highly sensitive hybrid GAsP/APD (HyD) for low light imaging. It has visible laser lines at 458nm, 488nm, 514nm, 561nm and 633nm ...

Welcome to the Confocal Microscopy Lab at RIT. This lab provides faculty and students with a multidisciplinary imaging research and training facility centered around confocal microscopy. The ...

The facility is composed of three parts: 1. Advanced light microscopy facility: two widefield microscopes for widefield imaging of samples and for long time lapse experiments of live cells, three ...

As microscopes have improved, so too has their resolution and their ability to image cellular substructure, even down to the single-molecule level. Researchers can now investigate the carefully ...