Out-of-focus light (red and blue dash lines in diagram) is eliminated because it follows a different light path and thus does not go through the pinhole. In laser-scanning confocal microscopy, a small ...

The basic scheme here is known as confocal laser scanning fluorescence microscopy, where a laser at one wavelength excites fluorescent tags bound to structures in a sample. Light emitted by the ...

The Leica SP8 is a dual purpose visible confocal microscope and multi-photon on a single platform. The confocal microscope is equipped with 4 detectors, one of which is a highly sensitive hybrid ...

Thus, as with image- scanning microscopy technologies, the Airyscan detection concept can be leveraged with multiphoton excitation to increase the imaging depth beyond that of traditional confocal ...