A calibration curve shown on the right graph was developed from standard diluted solutions of Food Green 3 using a least squares linear regression equation. Credit: Dr. Justin Tom. For data analysis, ...

Differences in Fluorescence and Absorption Methods of Measurement. The Beer-Lambert’s law may be used for determining the concentration of the molecules in a solution using UV-Vis absorption.

Open Excel and create a new spreadsheet. Enter the standard samples you are graphing into cells "A1" and "A2." For example, enter "Concentration" and "Absorbance." ...