Gel electrophoresis is a fundamental technique for separating molecules such as DNA, RNA and proteins in laboratories across the biological disciplines. But how does polyacrylamide gel electrophoresis ...

Two-dimensional polyacrylamide gel electrophoresis, or 2D PAGE, was introduced in 1975 concurrently by Joachim Klose 7 and Patrick H. O’Farrel. 8 In the first dimension, proteins are separated ...

Generally, the in-gel digestion methodology has become routine for proteins separated by 2-D electrophoresis while in-solution digestion is usually used in LC-MS/MS analysis. The protein is cut ...

For even more helpful information on how to run your gels, don’t miss GEN’s 20 Tips for Terrific 2D Gel Electrophoresis Results! To learn more about Bio-Rad and its life science products, go ...

Electrophoretic mobility shift assays (EMSAs) are commonly used to analyze nucleic acid–protein interactions. When nucleic acid is bound by protein, its mobility during gel electrophoresis is ...

Automating 2-D Gel Electrophoresis Courtesy of Amersham Biosciences Amersham Biosciences www.amershambiosciences.com The Ettan™ proteomics product line includes the Ettan IPGphor™ IEF system and Ettan ...

Analyze proteins better with these tips. Two-dimensional gel electrophoresis (2DE) is a uniquely powerful technique that allows separation of up to thousands of proteins while retaining pI and MW ...

Courtesy of Pierce Biotechnology Though well traveled, the road to two-dimensional gel analysis can be slow going. Pierce Biotechnology in Rockford, Ill., has looked to the past in developing four new ...